南京梅迪蒙生物
临检服务
Inspection service
慢病毒包装 Lentivirus Packaging
慢病毒包装
Lentivirus Packaging
慢病毒(Lentivirus)载体是以 HIV-1 (人类免疫缺陷1型病毒)为基础发展起来的基因治疗载体。具有感染谱广泛、可以有效感染分裂期和静止期细胞、长期稳定表达外源基因等优点,因此成为导入外源基因的有力工具。现在慢病毒系统已经被广泛应用到各种细胞系的基因过表达、RNA干扰、microRNA研究以及活体动物实验中。
慢病毒特点
感染范围广
慢病毒可有效感染分裂和非分裂细胞,适合几乎所有细胞系,如神经元细胞、肝细胞、心肌细胞、肿瘤细胞、内皮细胞、干细胞等。
可稳定表达
慢病毒可将外源基因整合到宿主细胞基因组上,不随着细胞的分裂传代而丢失,可实现目的基因的长时间稳定表达。
操作安全性高
慢病毒采用的是自失活复制缺陷型病毒株,保障操作安全。
慢病毒产品与服务
服务类型提供产品滴度适用范围
常规基因过表达/干扰慢病毒
miRNA sponge/antago慢病毒
circRNA /LncRNA过表达和干扰慢病毒
CRISPR/Cas9慢病毒定制
慢病毒稳定株筛选
慢病毒现货
常规滴度慢病毒108TU/ml适用于普通细胞系感染
高滴度慢病毒109TU/ml适用于难转染细胞系,如悬浮细胞;或用于动物活体水平感染

服务流程


质量标准
检测项目检测方法检测结果
支原体PCR阴性
内毒素LAL合格
细菌培养法阴性
真菌培养法阴性
活性滴度感染法≥10^8 TU/ml
梅迪蒙优势

1、专注提供病毒包装服务十余年

2、上万篇SCI期刊引用,包括CELL、Science等

3、一年有10000+客户和汉恒合作,项目经验10W+

4、ISO9001认证的质量体系,严格的病毒纯化工艺

5、以客户为中心的强大售后服务体系,准时交付率连续三年超98%

6、完善的客户服务体系,技术支持、项目跟进、售后服务获得客户一致好评,确保您订购无忧

载体选择(常用)
编号调控方式元件顺序原核抗性真核抗性荧光标记启动子
LV005过表达pHBLV-CMV-MCS-EF1-mCherry-T2A-PuromycinAmpicillinPuromycinmCherryCMV
LV006过表达pHBLV-CMV-MCS-3flag-EF1-mCherry-T2A-PuromycinAmpicillinPuromycinmCherryCMV
LV007过表达pHBLV-CMV-MCS-EF1-PuromycinAmpicillinPuromycinCMV
LV008过表达pHBLV-CMV-MCS-3flag-EF1-PuromycinAmpicillinPuromycinCMV
LV011过表达pHBLV-CMV-MCS-3flag-EF1-ZsGreen-T2A-PuromycinAmpicillinPuromycinZsGreenCMV
LV012过表达pHBLV-CMV-MCS-EF1-ZsGreen-T2A-PuromycinAmpicillinPuromycinZsGreenCMV
LV052过表达pHBLV-CMV-MCS-EF1-ZsGreenAmpicillinZsGreenCMV
LV05过表达pHBLV-CMV-MCS-3flag-EF1-ZsGreenAmpicillinZsGreenCMV
LV081过表达pHBLV-EF1-MCS-CMV-PuromycinAmpicillinPuromycinEF1
LV082过表达pHBLV-EF1-MCS-CMV-ZsGreenAmpicillinZsGreenEF1
LV083过表达pHBLV-EF1-MCS-CMV-ZsGreen-T2A-PuromycinAmpicillinPuromycinZsGreenEF1
LV121过表达pHBLV-CMV-MCS-3flag-EF1-Luc-T2A-PuromycinAmpicillinPuromycinLuciferaseCMV
LV122过表达pHBLV-CMV-MCS-EF1-Luc-T2A-PuromycinAmpicillinPuromycinLuciferaseCMV
LV027过表达tetonpHBLV-TetOn-SV40-Puromycin-TRE3GS-MCSAmpicillinPuromycinTRE3GS
LV019干扰pHBLV-U6-MCS-PGK-PuromycinAmpicillinPuromycinU6
LV020干扰pHBLV-U6-MCS-CMV-ZsGreenAmpicillinZsGreenU6
LV021干扰pHBLV-U6-MCS-CMV-ZsGreen-PGK-PuromycinAmpicillinPuromycinZsGreenU6
LV051干扰pHBLV-U6-MCS-EF1-mCherry-T2A-PuromycinAmpicillinPuromycinmCherryU6
LV124干扰pHBLV-U6-MCS-EF1-Luc-T2A-PuromycinAmpicillinPuromycinLuciferaseU6
LV144干扰PHBLV-U6-MCS-CMV-mCherryAmpicillinmCherryU6
LV050cas9/gRNApHBLV-U6-gRNA-EF1-CAS9-PuromycinAmpicillinPuromycinU6
LV055cas9/gRNApHBLV-U6-gRNA-EF1-ZsGreenAmpicillinZsGreenU6
LV133cas9/gRNApHBLV-U6-gRNA-EF1-ZsGreen-LucAmpicillinLuciferaseU6
慢病毒感染实例


客户发表文献节选
T lymphocyte membrane-decorated epigeneticnanoinducer of interferons for cancerimmunotherapy
(杂志:Nature Nanotechnology,IF=39.213,中国科学院上海药物研究所)
The circRNA circSEPT9 mediated by E2F1 and EIF4A3 facilitates the carcinogenesis and development of triple-negative breast cancer
(杂志:Molecular Cancer,IF=27.401,重庆医科大学)
CircPSMC3 suppresses the proliferation and metastasis of gastric cancer by acting as a competitive endogenous RNA through sponging miR-296-5p
(杂志:Molecular Cancer,IF=27.401,南京医科大学第一附属医院)
Targeting E2 ubiquitin-conjugating enzyme UbcH5c by small molecule inhibitor suppresses pancreatic cancer growth and metastasis
(杂志:Molecular Cancer,IF=27.401,浙江省肿瘤医院)
Circular RNA MTCL1 promotes advanced laryngeal squamous cell carcinoma progression by inhibiting C1QBP ubiquitin degradation and mediating beta-catenin activation pathway
(杂志:Molecular Cancer,IF=27.401,中国医科大学第一附属医院)
Extracellular vesicle‐mediated delivery of circDYM alleviates CUS‐induced depressive‐like behaviours
(杂志:Journal of Extracellular Vesicles,IF=25.841,东南大学)
Exosomes derived from osteogenic tumor activate osteoclast differentiation and concurrently inhibit osteogenesis by transferring COL1A1-targeting miRNA-92a-1-5p
(杂志:Journal of Extracellular Vesicles,IF=25.841,空军军医大学西京医院)
TRIB3 Interacts with Beta-catenin and TCF4 to Increase Stem Cell Features ofColorectal Cancer Stem Cells and Tumorigenesis
(杂志:GASTROENTEROLOGY,IF=22.682,北京协和医院)
HACE1-mediated NRF2 activation causes enhanced malignant phenotypes and decreased radiosensitivity of glioma cells
(杂志:Signal Transduction and Targeted Therapy,IF=18.187,西安交通大学)
TRIB3 reduces CD8+ T cell infiltration and induces immune evasion by repressing the STAT1-CXCL10 axis in colorectal cancer
(杂志:Science Translational Medicine,IF=17.956,中国医学科学院药物研究所)